The Use of Natural Products to Destabilise Biofilms?

There is relatively little information on specific control of Campylobacter biofilms, but more research has been done in the control of biofilms in other areas (such as the dental area) and a broad range of natural products are known to destabilise or reduce biofilms, herbal products such as Trigonella (Seed) Extract, [1] Chamaemelum Extract, [2] Tannins, [3] Thyme and Olive leaf extracts, [3] diallyl-sulphide, [5] bacteriophages, furanones that interfere with quorum sensing molecules, [6] bacteriocins, [7,8] a broad range of peptides, [9,10,11] alkaloids, [12] non-cariogenic sugars (Tagatose, [13] Xylitol [14])  and glycolipids. [15] DNA, proteins, and EPS constitute the biofilm matrix, and recent studies revealed that effective disruption of the biofilm architecture could be achieved by various enzymes. Matrix-degrading enzymes such as DNase, alginate lyase, [16] amylase [17] lactonase, [18] glucan hydrolase, [19] have all played a role in the degradation of biofilms from a range of different bacteria.

This feasibility project is expected to investigate the use of food-grade enzymes to specifically degrade Campylobacter biofilms, and examine the possible synergy of the approach with natural-food grade herbal extracts or actives. It will also determine if suitable grafting/coating of enzymes and herbal actives in food-packaging could allow the control of Campylobacter, both in the packaging itself and in poultry meat.

Figure 5- Model packaging to be developed in subsequent projects
Photo credit: Genuineideas.com

In a subsequent stage of packaging development, the data obtain in the present feasibility will feed into a design of a new anti-biofilm coating.
At this stage it is expected that such coating will impart multiple anti-Campylobacter effects (see Figure 5),

a) on the interface meat- packaging it will interact via enzymes grafted on the plastic that will cleave selectively the Campylobacter biofilm, while

b) the herbal extract release will act synergistically to improve the control of the pathogen.  

References

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  2. Kazemian H, Ghafourian S, Heidari H, Amiri P, Yamchi JK, Shavalipour A, Houri H, Maleki A, Sadeghifard N. Antibacterial, anti-swarming and anti-biofilm formation activities of Chamaemelum nobile against Pseudomonas aeruginosa.  Rev Soc Bras Med Trop. 2015 Aug; 48(4):432-6.
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